crispr guide tool Search Results


crispr guide verification tool  (Synthego Inc)
90
Synthego Inc crispr guide verification tool
Crispr Guide Verification Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr guide verification tool/product/Synthego Inc
Average 90 stars, based on 1 article reviews
crispr guide verification tool - by Bioz Stars, 2026-03
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crispr guide tool  (Benchling Inc)
90
Benchling Inc crispr guide tool
Crispr Guide Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr guide tool/product/Benchling Inc
Average 90 stars, based on 1 article reviews
crispr guide tool - by Bioz Stars, 2026-03
90/100 stars
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in silico crispr guide rna selection tool  (Benchling Inc)
90
Benchling Inc in silico crispr guide rna selection tool
Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2- deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2 -wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2 flox/flox parental and Setd2 flox/flox ; Nsd1 −/− MEF cell lines. G Crystal violet staining of Setd2 flox/flox and Setd2 flox/flox ; Nsd1 −/− MEF cell lines following treatment with 4-OHT
In Silico Crispr Guide Rna Selection Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in silico crispr guide rna selection tool/product/Benchling Inc
Average 90 stars, based on 1 article reviews
in silico crispr guide rna selection tool - by Bioz Stars, 2026-03
90/100 stars
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crispr guide ranking tool  (Benchling Inc)
90
Benchling Inc crispr guide ranking tool
Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2- deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2 -wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2 flox/flox parental and Setd2 flox/flox ; Nsd1 −/− MEF cell lines. G Crystal violet staining of Setd2 flox/flox and Setd2 flox/flox ; Nsd1 −/− MEF cell lines following treatment with 4-OHT
Crispr Guide Ranking Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr guide ranking tool/product/Benchling Inc
Average 90 stars, based on 1 article reviews
crispr guide ranking tool - by Bioz Stars, 2026-03
90/100 stars
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eukaryotic pathogen crispr guide rna/ dna design tool  (Becton Dickinson)
90
Becton Dickinson eukaryotic pathogen crispr guide rna/ dna design tool
Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2- deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2 -wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2 flox/flox parental and Setd2 flox/flox ; Nsd1 −/− MEF cell lines. G Crystal violet staining of Setd2 flox/flox and Setd2 flox/flox ; Nsd1 −/− MEF cell lines following treatment with 4-OHT
Eukaryotic Pathogen Crispr Guide Rna/ Dna Design Tool, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eukaryotic pathogen crispr guide rna/ dna design tool/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
eukaryotic pathogen crispr guide rna/ dna design tool - by Bioz Stars, 2026-03
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crispr guide tool crispick93  (Broad Institute Inc)
90
Broad Institute Inc crispr guide tool crispick93
Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2- deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2 -wildtype/mutant HAP1 cells. B Schematic depiction of <t>CRISPR/Cas9</t> synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2 flox/flox parental and Setd2 flox/flox ; Nsd1 −/− MEF cell lines. G Crystal violet staining of Setd2 flox/flox and Setd2 flox/flox ; Nsd1 −/− MEF cell lines following treatment with 4-OHT
Crispr Guide Tool Crispick93, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crispr guide tool crispick93/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
crispr guide tool crispick93 - by Bioz Stars, 2026-03
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Image Search Results


Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2- deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2 -wildtype/mutant HAP1 cells. B Schematic depiction of CRISPR/Cas9 synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2 flox/flox parental and Setd2 flox/flox ; Nsd1 −/− MEF cell lines. G Crystal violet staining of Setd2 flox/flox and Setd2 flox/flox ; Nsd1 −/− MEF cell lines following treatment with 4-OHT

Journal: Genome Biology

Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation

doi: 10.1186/s13059-025-03483-z

Figure Lengend Snippet: Unbiased genome-wide screening identifies NSD1 as putative SL modifier in SETD2- deficient cells. A Western blot analysis of global H3K36 methylation states in isogenic SETD2 -wildtype/mutant HAP1 cells. B Schematic depiction of CRISPR/Cas9 synthetic lethal screen. C Volcano plot highlighting NSD1 as a synthetic lethal hit (SL index: − 1.76; p value = 2.67e − 06). D Gene ontology analysis of the 127 SL candidates identified in the screen reveal enrichment for factors involved in epigenetic remodeling and DNA damage/repair. E Gene-view schematic illustrating inducible deletion of Setd2 in MEFs through Cre-lox excision of exon 6. F PCR genotyping confirming tamoxifen-inducible Cre activity in the Setd2 flox/flox parental and Setd2 flox/flox ; Nsd1 −/− MEF cell lines. G Crystal violet staining of Setd2 flox/flox and Setd2 flox/flox ; Nsd1 −/− MEF cell lines following treatment with 4-OHT

Article Snippet: Individual sgRNAs for CRISPRi targeting were selected using an in silico CRISPR guide RNA selection tool (Benchling) with corresponding oligos annealed and subcloned by cohesive-end ligation into a lentiviral mU6-(sp)TRACR guide RNA vector following AarI digestion.

Techniques: Genome Wide, Western Blot, Methylation, Mutagenesis, CRISPR, Activity Assay, Staining